Understanding the Impact of Vial Dead Volume on Injection Precision

Tips and Tricks for HPLC and UHPLC - Thermo Fisher Scientific

• Do not overfill the vial – The septum is used to clean the needle. Fill 2/3 of the volume. • If you use inserts make sure there are no airbubbles in them. • Autosamplers with PEEK™ (Victrex Plc.) needle must have caps with slit septa. • Never shake the vial. If you do there is going to be sample

SPME Troubleshooting Guide - Bulletin 928 - MilliporeSigma

Splitless injection is required with SPME to focus the analytes on the chromatographic column. Close the splitter vent valve for at least two minutes during sample injection. Narrow-bore inlet liners (0.75 mm I.D.) will reduce peak broad-ening in the chromatographic analysis by minimizing the dead volume during analyte transfer onto the column.

HPLC Troubleshooting Guide - MilliporeSigma

Inject smaller volume (e.g., 10 μL vs. 100 μL) or inject the same volume after 1:10 or 1:100 dilutions of sample. Adjust solvent. Whenever possible, inject samples in mobile phase. Substitute new column of same type to confirm column as cause. Discard old column if restoration procedures fail.

Practical Steps in GC Troubleshooting - Aijiren

- dead volume, obstruction, poor installation, or severe column contamination Miscellaneous - overloading of PLOT columns, co-elution, polarity mismatch between phase, solute or solvent, and some compounds always tail *Tip = Inject a light hydrocarbon, should not tail unless flow path problem.

LOW DEAD-VOLUME (LDV) SYRINGES AND/OR NEEDLES GUIDE

Use of low dead-volume (LDV) syringes and/or needles may maximize the potential number of vaccine doses and minimize vaccine wastage2. Low dead-volume (also called low dead-space) is the amount of fluid remaining within the syringe and needle after an injection is completed3.

Allowable Excess Volume and Labeled Vial Fill Size in

Mar 14, 2014 · 60 for drugs in ampules or vials that are intended for injection, the declaration of net quantity of 61 contents on the label is considered to express the minimum quantity of contents and further

Moderna COVID-19 Vaccine Health Care Provider Fact Sheet

o If the vial stopper has been punctured 20 times, discard the vial and contents. o If the amount of vaccine remaining in the vial cannot provide a full dose of 0.5 mL or 0.25 mL, discard the vial

Troubleshooting Peak Area Variation Between Injections

Nov 10, 2022 · Aijiren Knowledge Portal More Troubleshooting Peak Area Variation Between Injections This Information Applies To: All Aijiren HPLC Autosampler modules Issue: Nonreproducible peak areas between injections, using the same sample and the same experimental conditions. Resolution:

Container Closure and Delivery Considerations for

Mar 11, 2021 · Delivering less than the target volume may lead to under dosing which will impact the efficacy and, in the case of some drugs, may impact the frequency of injection as well. Injection volume is usually based on the intended dose and concentration of a product. IVT injection volumes are typically between 0.05 and 0.1 mL.

the illusion of accuracy - American Society of Health-System

Mar 5, 2019 · Below 50% of its nominal volume, the accuracy of the syringe declines with the volume measured until, at 10% of its nominal volume, it delivers ±16% accuracy. So when measuring a volume of 0.1 mL in a 1 mL syringe, the best you can actually know is that you are delivering somewhere between 0.084 and 0.116 mL.

Experimental Considerations in Headspace Gas Chromatography

May 2, 2010 · This gas phase is the headspace, which is sampled and injected onto the column using pressure as the injection mode. The injection precision of a headspace injector can consistently give < 10.0% relative standard deviation (RSD) and frequently gives ≤ 2.0% RSD, similar to the precision found with liquid-chromatography injection.

Reproducibility of GC Analysis Techniques for Improving the

Inlet Liners – Volume Considerations Glass Inlet Liners provide an “inert” space for liquid samples to be uniformly vaporized to a gas and moved to the column. Liquid-gas phase change involves a significant change in volume. Gaseous sample volume depends on • Injection volume • Solvent type • Column head pressure • Inlet temperature

Increasing vaccine supply with low dead-volume syringes and

Oct 10, 2021 · The average injected volume with or without injection of this dead volume was 1.826 ± 0.003 mL and 1.790 ± 0.002 mL respectively (Fig. 1-B). In contrast, these experiments revealed no inter- and intra-operator differences on the injection of the dilution volume ( Fig. 1 -C).

Container Closure and Delivery Considerations for - Springer

Mar 11, 2021 · Delivering less than the target volume may lead to under dosing which will impact the efficacy and, in the case of some drugs, may impact the frequency of injection as well. Injection volume is usually based on the intended dose and concentration of a product. IVT injection volumes are typically between 0.05 and 0.1 mL.

Troubleshooting and Diagnostics Tips and Tricks

Loss of precision Incorrect peak integration Check loss of precision is for all peaks in the cromathogram. –If it is only for some of them does not seem a injector problem Check if reproducibility lack is for areas or also for Retention times. –Check for leaks ©2013 Waters Corporation 15 Check injection volume and sample concentration