The microtitre plate was removed from the Triversa Nanomate and placed in the autosampler of the HPLC system (Ultimate 3000; Dionex, Thermo Fisher Scientific, Loughborough, UK), which is coupled to a Thermo Fisher Orbitrap Velos ETD mass spectrometer (Thermo Fisher Scientific, Bremen, Germany) via the Triversa Nanomate. .. Five μL aliquots