The Role of Vial Dimension in Minimizing Dead Volume Effects in HPLC

Filter Vial Overview

Hplc Vial: HPLC Cap: Spin Filters: High Recovery Vials: HPLC Inserts: Fill & Dead Volume: Fill: 450μL / Dead:120μL: Fill: 250μL / Dead:10μL: Pre Filtration: Particulate-Laden Samples: Minimize Matrix Effects & Ion Suppression: Available with pre-slit or non-slit caps: 10µL sample for 2μL injection: evaporation rate: <0.4% / 24hrs *

Influence of HPLC-System dead volume on the - Tosoh

The data clearly shows, that the extra column dead volume drastically influences column performance. When using a not optimized system it is recommended to use a larger inner diameter column. This way the time the analyte spends in the extra column volume is decreased and the extra column volume compared to the column volume becomes smaller.

Vanquish Flex UHPLC System | Thermo Fisher Scientific - US

Injection volume range: Standard: 0.01 - 25 µL. Optional: 0.01 - 100 µL. Sample cooling: 4-40°C: Sample capacity (standard) Based on standard 12 mm OD vials (≤1.5 mL) 4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available + capacity of 12 × 22.5 mm OD vials (≤10 mL) in the carousel. Sampler extension available (optional)

High Performance Liquid Chromatography - Chemistry LibreTexts

Aug 29, 2023 · The HPLC detector, located at the end of the column, must register the presence of various components of the sample, but must not detect the solvent. For that reason there is no universal detector that works for all separations. A common HPLC detector is a UV absorption detector, as most medium to large molecules absorb UV radiation. Detectors

HPLC Basics | Thermo Fisher Scientific - US

High-performance liquid chromatography (HPLC) is a broad analytical chemistry technique used to separate compounds in a chemical mixture. These separations utilize the pressure-driven flow of a mobile phase through a column packed with a stationary phase. The mobile phase carries a liquid sample through the column to the detector, and compounds

3.2: High Performance Liquid chromatography - Chemistry

3.2: High Performance Liquid chromatography. High-performance liquid chromatography (HPLC) is a technique in analytical chemistry used to separate the components in a mixture, and to identify and quantify each component. It was initially discovered as an analytical technique in the early twentieth century and was first used to separate colored

Vanquish Horizon UHPLC System | Thermo Fisher Scientific - US

Injection volume range: Standard: 0.01-25 µL Optional: 0.01-100 µL: Sample cooling: Yes, 4-40°C. Sample capacity (standard) Based on standard 12 mm OD vials (≤ 1.5 mL) 4 racks of 54 vials (≤ 1.5 mL) each, other rack formats available + capacity of 12 × 22.5 mm OD vials (≤ 10 mL) in the carousel. Sampler extension available: Yes, with

Lab 2: High Performance Liquid Chromatography - Chemistry

Fill a vial with the appropriate volume and label the vial. 3. Prepare the Tea Sample. a. Pipette 10 mL of tea into a clean and Dry 50 mL volumetric flask and dilute to the mark with HPLC/CE grade water. b. Filter the sample using the provided filter. c. Rinse the filter by filtering the first 1-2mL of the sample into the waste beaker. d.

How Much Can I Inject? Part I: Injecting in Mobile Phase

Oct 1, 2014 · From this, we can see that for a 1% loss in resolution, Vs ≈ 0.15 Vp0. That is, if we inject using mobile phase as the injection solvent, we can inject up to ~15% of the volume of the first peak of interest, which I call the 15% Rule. Similarly, if we can tolerate a 5% loss in resolution, the factor is ~30% of Vp0, and for a 10% loss, ≈40%

Autosampler Vials & Caps for HPLC & GC | Thermo Fisher

These one-piece microvials have a very low residual volume due to a specially shaped deep and narrow inner reservoir which collects the liquid in the base so compatible autosamplers can collect all but 1-2 microliters of sample while having a total sample volume of 1.2mL. The outer dimension for these vials is 12x32mm.

High Recovery Vials & Vial Inserts, HPLC Recovery Vials | Aijiren

Aijiren's broad range of high recovery vials (also referred to as high recovery HPLC vials) are the vial of choice when working with limited sample volumes but not wanting to manually combine a standard 2 mL vial with a separate vial insert. Our portfolio also includes HPLC vial inserts that can be used with any 2mL vial for limited volume

Sample Vials and Accessories - Waters Corporation

incinerated while still sealed, minimizing exposure to potentially hazardous substances. The maximum temperature use is 135 ˚C. The Waters Vials Selector is designed to simplify the process of selecting the best vial solutions for your system and application requirements. For more information about choosing the correct vial for your application,

Sample Vials | Waters

Part Number: 186000846C. Waters Certified vials are tested for cleanliness by HPLC and held to tightest dimensional tolerances in the industry. LC GC Amber Glass 12 x 32mm Screw Neck Vial, with Cap and PTFE Septum, 2 mL Volume, 100/pkg. Part Number: 186000846C. ----.

What Is “Dead” Volume and Why Should Chromatographers Worry

Nov 1, 2015 · The dead volume of a chromatography system is defined by the International Union of Pure and Applied Chemistry (IUPAC) (1) as an obsolete term equal to the mobile-phase volume that solutes experience outside of the column, equivalent to the extracolumn volume. Unfortunately, there is a good deal of confusion about this definition because the

Evaluating the Impact of LC System Dispersion on the Size

Extra-column dispersion can be considered as the increase in the volume of an injected sample that occurs as it travels through an LC system’s flow-path without a column. Consequently, as the volume of this dispersion increases in proportion to the volume of the separated peaks, the resolution of the SEC separation will diminish.