Oct 10, 2013 · The role of the pump is to propel (force) a liquid (the mobile phase) through the chromatograph at a specific flow rate, expressed in ml/min. Normal flow rates in HPLC are 1-2 ml/min and typical pumps can reach pressures in the range of 2000 – 9000 psi but in applications covered under UHPLC mode operating pressure can be as high as 15000-18000 psi.
Figure 2. Chromatogram of a polymer under investigation. This separation is based on a slight difference in hydrophobicity between the mono and difunctional species. Figure 2 shows the chromatogram of a polymer under investigation. In this case, the minor component is not detected, illustrating that this HPLC technique can be used
High-performance liquid chromatography, or HPLC, is a highly versatile technique that separates components of a liquid mixture based on their different interactions with a stationary phase. HPLC is an adaptation of column chromatography. In column chromatography, a column is packed with micro-scale beads called the stationary phase.
High Performance Liquid Chromatography (HPLC) is an analysis technique used for separation and quantification of small molecules and polymers. HPLC is the method of choice for testing complex mixtures containing non-volatile and semi-volatile organic compounds such as polymer additives , active pharmaceutical ingredients (API’s), and impurities.
2. Standard narrow neck design for GC and HPLC. 3. 8mm wider opening for easy puncture. 4. 8mm PTFE/silicone septa, has excellent chemical inertness, acid and alkali resistance. 5. 8mm screw black open top pp caps. Technical Parameter. 1. 8-425 Thread Small Opening Vials ND8
1.5ml Snap Top Vials ND11, wide opening hplc vial with matched PE Snap Top Seals ND11, PTFE/rubber septa & Micro-Insert. Vials with integrated Micro-Insert are available ; Vials have a 40% larger opening than standard aluminum seal vials for easier sample filling and to reduce the chance of bent or broken needles during sampling.
EPRUI Biotech Co. Ltd. offers a new generation of reversed phase polymer HPLC packing material. Our polymer chromatography column packing material is made of highly crosslinked monodisperse polymer resins including highly crosslinked polystyrene divinylbenzen, polymethyl methacrylate, copolymers of PS/DVB and PMMA.
conventional mandrel and reverse-flow check valve, the J-40 and J-20 series valves can be used for either tubing or annular flow. Port sizes The port sizes available for the ˚⁄˝ -in [15.9-mm] OD J-50 valves are ˙⁄˝, ˚⁄ˆˇ, ˙˘⁄ , and ˙⁄ in [3.2, 3.9, 5.1, and 6.4mm]. Port sizes are available for the 1-inch [25.4-mm] OD J-40
Organic solvents used in normal-phase separations are more Mass Spectroscopy (MS) friendly than some of the typical buffers used in HPLC. In reversed-phase HPLC, operating conditions sometimes must be dramatically modified to allow compatibility with MS detection. Reversed Phase The inverse of normal phase chromatography.
Aminex HPLC columns, which are packed with a polymer-based matrix, are the research and industry standard for the analysis of carbohydrates, alcohols, and organic acids in food and beverage, biochemical, biofuel, biomedical, and biotechnology applications. These columns allow the use of simple isocratic
Several styles are offered including flat bottom, concial inserts with a top spring or a bottom spring, and concial without springs. Glass inserts are sold in two diameters for 2mL vials; one for standard opening (4.6 mm) vials and another for large opening (6 mm). The volume will vary depending on the length and the style of insert.
V9491P, Pack, Insert+1.5ml, Vial+Cap+Septa, Screw, Clear. V4091 - 250uL Insert with mandrel interior and polymer feet, 29*5.7mm, for 9mm Vials, 100ea/pkgV917 - 1.5ml wide opening short screw-thread vial, clear 11.6*32mm borosilicate type 70, 100ea/pkg C9191 - White PTFE/red silicone septa, 9mm blue short screw-thread polypropylene closure, 6mm centre hole, 100ea/pkg ..
HPLC column: This is the mandatory HPLC part on which actual separation of compounds occur. The HPLC columns have fixed lengths, diameter and also the particle size of the stationary phase to choose based on our requirement. The longer columns are used for separation of the complex mixture with many components.
Immediately find HPLC and UHPLC columns for reversed phase, chiral, normal phase, GFC, GPC, ion exchange, HILIC, and SFC using our HPLC product search.
The HPLC Manifold Kit, designed for use with Justrite FM Approved Quick Disconnect Safety Cans, enables up to four waste lines from HPLC processes to be fed into a single intake fitting. Kit includes enough fittings to accommodate tube lines either 1/16" (1.6 mm) OD or 1/8" (3.2 mm) OD.
