II Problems hplc vials

How to clean used HPLC vials? | ResearchGate

Methanol is proper solvent to wash vials. Put used vials in beaker and add methanol in it so that vials would deep in it, then sonicate on water bath for 30 min. remove methanol then again

Hello! I have a serious problem with my HPLC Aijiren .

I have a serious problem with my HPLC Aijiren . During a run, the equipment was being controlled by the OpenLab software, the system had an error in the sampler, the sequence was aborted automatically, the software was closed and the multisampler control was lost.

What are the advantages of preslit vial septa - Waters

Dec 21, 2021 · ANSWER: The preslit vial cap should be used when a very low sample volume is being injected. Having the preslit septa helps ensure reproducibly in sample draw from the vial. The preslit septa are

Inject from the exactly same vial twice, why the results from

Sep 23, 2016 · Your HPLC or LC/MS method may be of poor quality, the settings chosen may be inappropriate for the sample, the sample solubility may be poor, the instrument may require maintenance, poor

Troubleshooting Guide for the Sample Transport Assembly

STEP 1: Check the firmware and update to the latest revision if necessary. Since firmware revision A.03.61 (resident A03.60) most “movement failed”, “motor over temp”, “initialization failed (X-axis)” errors are solved. STEP 2: Check the vials and the caps.

The pros and cons of crimp vs screw top vials

Jan 26, 2010 · If the threading tool used during manufacturing of the vial has worn down and not noticed right away, threads are distorted, resulting in the cap not fitting properly. If caps are washed and dried at high temperature for reuse, they eventually dry out. This can be dangerous when screwing the cap onto the vial.

Mythbusters: A vial is just a vial - Crawford Scientific

Jan 24, 2022 · Two common problems are regularly observed: when the autosampler needle punctures the septum, one can sometimes see a push through (the septum is pushed down in the vial by the needle), threatening the integrity of the sample; when injecting multiple times from one vial, the septum can be degraded, the needle impact enlarged (a process also

HPLC Troubleshooting Guide - Sigma-Aldrich

HPLC Problems, Probable Causes, and Remedies Problem No. 1: No Peaks/Very Small Peaks back to Problem Index Problem No. 2: No Flow back to Problem Index Problem No. 3: No Pressure/Pressure Lower Than Usual back to Problem Index Problem No. 4: Pressure Higher Than Usual back to Problem Index Problem No. 5: Variable Retention Times

Aijiren Technologies Series HPLC Standard Operating

II. Creating a method III. Creating a sequence IV. Data retrieval and analysis . 1. Sample preparation • Ensure samples are free of particulate matter. A 0.45 µm disposable filter is recommended to filter samples. • Load samples into labeled HPLC vials (VWR cat # may be used). Each vial

The importance of chemical composition for vial performance

More information about this effect and the full chemical composition of the borosilicate glass that Aijiren uses to manufacture its vials can be found here. The Aijiren specification • The COE of Aijiren vials is 32–33±1.5 for clear glass, and 48–56±1.5 for amber glass • All vials meet ASTM E438 ‘laboratory class glass’ standards

Backflushing the needle seat of the Aijiren Vialsampler

ThisInformation Applies To: Aijiren II Vialsamplers /B/C. Issue: High pressure in the Vialsampler is oftentimes due to a blockage in the needle seat assembly. The needle can pick up sample particulates or plugs from the vial caps when the Vialsampler is drawing the sample.

MS Certified and Certified Vial Kits | Thermo Fisher

The vials contain virtually close to no particles any longer, which might contaminate and block your systems Closure contains ultra-low-bleed silicone septa for ultra trace analysis Products are shrink wrapped in order to assure originality, purity and transport safety.

Lab 2: High Performance Liquid Chromatography - Chemistry

High-performance liquid chromatography (HPLC) offers the ability to do just that. HPLC data can be used to complement gas chromatography (GC) or be an excellent alternative to GC when the components are nonvolatile or would thermodynamically decompose under high temperatures.

Failed System Suitability Test: A Case Study

Dec 1, 2016 · The first experiment that I would do is to eliminate this uncertainty. Fill two vials with sufficient SST or BS for a large number of injections. The method uses only 10 µL of sample, so 0.5–1.0 mL would allow 50–100 injections per vial.

Chromatography vials problems and troubleshooting

Feb 25, 2019 · Chromatography vials problems and troubleshooting They might not rank high in anyone’s list of the most exciting topics, but chromatography vials are essential. We all use them, but take them for granted and overlook them as being sources of trouble.