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Heat-denature the RNA samples to be analyzed at 70°C for 2 minutes. Immediately cool to 4°C and keep on ice before use. 8.1.17. Using a fresh RNase-free 96-well Eppendorf twin.tec sample plate, pipette 18 µL of the High Sensitivity RNA Diluent Marker (15nt) (DM) Solution to each well that is to contain sample or RNA Ladder.
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