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Jun 04, 2020 · Last, the pellet was sterile filtered through a 0.45 µm polyvinylidene fluoride syringe filter (Thermo Fisher Scientific, Waltham, MA; Cat. No. 09-720-4) into a sterile tube. The rosette-inducing activity of the OMVs was tested by serially diluting the purified OMVs in a 24-well plate, with each well containing 0.5 ml of S. rosetta at a
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Consumption of vegetables represents a route of direct human exposure to bacteria found in soil. The present study evaluated the complement of bacteria resistant to various antibiotics on vegetables often eaten raw (tomato, cucumber, pepper, carrot, radish, lettuce) and how this might vary with growth in soil fertilized inorganically or with dairy or swine manure.
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The same cell lines as in (B) were transfected with the indicated combinations of RPN1-HA and/or UFM1-ΔCS for 24 h. Cells were then lysed for immunoprecipitation of HA epitope. Samples were resolved using 3-(N-morpholino)propanesulfonic acid (MOPS) buffer for better molecular weight separation between unmodified and UFMylated RPN1 proteins.
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