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ACME C18 Columns Retention time reproducibility and column stability are shown on the ACME column in the figure below. The figure compares the chromatograms shown from the 1st injection compared to
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Inventive concepts relate generally to the field of cell culture, and more particularly to formation of three-dimensional aggregate(s) of eukaryotic cells. Cell culture vessels including a conical device having an inner surface being hydrophobic; and a frustum defining an open viewing aperture and a narrow end of the conical device are described.
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2) add 150ul cells to DNA samples in 13ml tubes on ice 3) inc 25' on ice with occassional mixing 4) heat shock 5', 37 C 5) inc ice 5' 6) add 1ml LB ‰without antibiotics_, shake 1hr 37 C 7) spin 30', asp to 200ul, plate 100ul, store the rest For blue/white screen, add IPTG and X-Gal to plates before starting transformation
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