----- 7 3 3 If the sample being tested requires more extensive dilution than is provided by Table 1, an intermediate dilution is prepared from 20 ml of sample diluted to 200 ml with odor-free water Use this dilution for the threshold determination Multiply the T O N obtained by ten to correct for the intermediate dilution In rare cases more
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of RNase-free water and mixing well. Divide the diluted ladder solution into aliquots with working volume typical for one day use or one sample plate. Store aliquots in the provided Eppendorf LoBind® 0.5 mL tubes at -70°C or below. 8.1.16. Heat-denature the RNA samples to be analyzed at 70°C for 2 minutes.
transfer remaining liquid into 5X vol. particle free DI water to dilute Percoll/sucrose (use 250mL or 500 mL conical tube). If the packed sediment in bottom of tube or bottle represents a significant portion of the floated sample (>50 uL/100 gallons), examine at least one slide directly (wet mount) or re-float as in section 10.6.2. Uncontrolled
