High Performance Liquid Chromatography (HPLC) is an analysis technique used for separation and quantification of small molecules and polymers. HPLC is the method of choice for testing complex mixtures containing non-volatile and semi-volatile organic compounds such as polymer additives , active pharmaceutical ingredients (API’s), and impurities.
Approximately 150 samples were analyzed over a 1-month period, providing fast (24-hour) turnaround and high quality results to the OSC. Mobile-analytical-laboratory support provided fast turnaround on high-quality analyses of several critical pollutants to assist the OSC in the assessment and characterization of site contamination.
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While HPLC can produce extremely high quality (pure) products, it is not always the primary method used in the production of bulk drug materials. According to the European pharmacopoeia, HPLC is used in only 15.5% of syntheses. However, it plays a role in 44% of syntheses in the United States pharmacopoeia.
Development of HPLC columns has began around 1980 and resulted in the development of a series of polymer-based columns which have turned into commercial products, including analysis SEC columns, preparative SEC columns, sugar and organic acid analysis columns, aqueous SEC columns, reverse-phase partition columns, and ion chromatography columns.
HPLC Stationary phases for a broad range of separations. For more than 35 years, Hamilton Company has developed and manufactured pressure-stable, polymeric polystyrene-divinylbenzene (PS-DVB) HPLC columns that are used in most of the world’s top chromatography labs.
HPLC stands for High Performance Liquid Chromatography. Before HPLC was available, LC analysis was carried by gravitational flow of the eluent (the solvent used for LC analysis) thus required several hours for the analysis to be completed. Even the improvements added in later time were able to shorten the analysis time slightly.
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May 23, 2011 · Figure 2. Chromatogram of a polymer under investigation. This separation is based on a slight difference in hydrophobicity between the mono and difunctional species. Figure 2 shows the chromatogram of a polymer under investigation. In this case, the minor component is not detected, illustrating that this HPLC technique can be used
With over 40 years as a leader in LC column technology, you can rely on the quality of Thermo Scientific™ HPLC and UHPLC columns. Our extensive family of products offers a variety of particle sizes and column designs to meet all separation needs, including improved resolution, enhanced sensitivity, faster analysis and consistent performance.
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