polymers. High Performance Liquid Chromatography (HPLC) is the techique of choice to measure free acrylic monomers in polymers before cross-linking. Typically, sample preparation to extract a variety of residual monomers from the polymer matrix is complex, tedious and time-consuming2,3,4. If the extract contains both polar and non-polar monomers
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HPLC stands for High Performance Liquid Chromatography. Its earlier name was High Pressure Liquid Chromatography because it involved use of liquid mobile phase requiring higher pressures than gases used in Gas Chromatography.
This typically ensures the proper interaction of analytes with the non-polar, hydrophobic particle surface. A C18–bonded silica [sometimes called ODS] is the most popular type of reversed-phase HPLC packing. Table C presents a summary of the phase characteristics for the two principal HPLC separation modes based upon polarity.
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Sep 12, 2018 · HPLC Basics High performance liquid chromatography or commonly known as HPLC is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy. A computer analyzes the data show the output in display. HPLC
