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To ensure a high RNA concentration, use the eluate to repeat the elution by reapplying to the membrane and spinning at ≥8000xg for 1 min.* Determine the concentration and quality of the RNA sample.* To reverse transcribe template RNA into cDNA, prepare the following reaction in one eppendorf: Up to 2 µg RNA* 4 µl of 10X Buffer RT
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Module 9 : Applications of High Performance Liquid Chromatography After having gained exposure to High Performance Liquid Chromatography systems and their components we now introduce to typical applications. HPLC has contributed to analytical solutions in diverse fields such as pharmaceuticals, foods, life sciences, environment, forensics, etc.
The recovery of low quality control (LQC) level was 76.56%, medium quality control (MQC) level was 78.29% and for high quality control (HQC) the level was 67.86%. The mean recovery of all the three QC's levels was 74.24% with the precision 7.53% C.V. Recovery of internal standard was 68.43%.
A precolumn filter is essential for protecting HPLC columns against particulate matter which can accumulate on the column frit, leading to split peaks and high back pressure. Sources of particles include mobile phases (especially when buffers are mixed with organic solvents), pump and injector seals, and samples.
Fitting thread size chart is intended as a quick reference guide for thread size by dash size.
recommended for HPLC use. Ultra pure HPLC water (18M Ω resistivity) is generated by passing deionized water through an ion exchange bed. Modern water purification instruments use this mechanism to produce water of suitable quality in high volumes. Alternately, HPLC grade water can be purchased from solvent suppliers. I-2. Buffers
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•HPLC is an abbreviation for High Performance Liquid Chromatography (It has also been referred to as High Pressure LC) •HPLC has been around for about 35 years and is the largest separations technique used •The history of HPLC: •Beginning of the 60’s: start of HPLC as High Pressure Liquid Chromatography
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Sep 12, 2018 · HPLC Basics High performance liquid chromatography or commonly known as HPLC is an analytical technique used to separate, identify or quantify each component in a mixture. The mixture is separated using the basic principle of column chromatography and then identified and quantified by spectroscopy. A computer analyzes the data show the output in display. HPLC
