HPLC, column section measurements are 2.1 mm to 4.6 mm distance across, and 30 mm to 250 mm length. Additionally, HPLC segments are made with smaller sorbent particles (2 μm to 50 μm in normal molecule size). This gives HPLC high determining or resolving power (the capacity to recognize components) while isolating mixtures,
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Jan 10, 2018 · HPLC, also known as High-Performance Liquid Chromatography system, is one of the most crucial analytical methods in pharmaceuticals. The purpose of HPLC is the separation of each compound that make up a mixture. It passes pressurized liquid solvent with the use of pumps which holds the sample mixture via a column full of solid adsorbent substance.
A precolumn filter is essential for protecting HPLC columns against particulate matter which can accumulate on the column frit, leading to split peaks and high back pressure. Sources of particles include mobile phases (especially when buffers are mixed with organic solvents), pump and injector seals, and samples.
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Oct 10, 2013 · The role of the pump is to propel (force) a liquid (the mobile phase) through the chromatograph at a specific flow rate, expressed in ml/min. Normal flow rates in HPLC are 1-2 ml/min and typical pumps can reach pressures in the range of 2000 – 9000 psi but in applications covered under UHPLC mode operating pressure can be as high as 15000-18000 psi.
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